Title:Development of Sensitive Optical Biosensing Technologies for Biomedical Applications

Time:16:00 pm  June 22, 2017

VenueZheng Yutong Lecture Hall, New Science Building

Speaker:Jingyong Ye,Department of Biomedical Engineering, the University of Texas at San Antonio

Abstract:

There is a high demand for highly sensitive biosensors for quantifying biomolecule concentrations or binding affinities for a wide range of biomedical and pharmaceutical applications. In this talk I will present two unique biosensors for both in vitro and in vivo applications. First, I will describe a photonic crystal based biosensor for label-free bioassays. As opposed to a conventional optical microcavity sensor that has a closed structure with its cavity layer sandwiched between two highly reflective surfaces, the photonic crystal sensor we developed possesses a novel open optical microcavity. The open configuration allows the sensing layer to be easily functionalized and directly exposed to analyte molecules for label-free bioassays through monitoring the change in resonant conditions of the open microcavity. A wide range of applications can be facilitated by the unique open-microcavity biosensor. In particular, two different applications will be covered in this talk. One is for label-free measurements of cardiac biomarkers- Troponin I (cTnI). The sensor is functionalized with antibodies against cTnI for sensitive detection of cTnI with a concentration as low as 0.1 ng mL-1. The other application is to use the sensor for the study of interactions between different peptides and FvTox1- a toxin produced by a soil borne pathogen that causes sudden death syndrome in soybean plants. Furthermore, I will discuss the development of a novel two-photon optical fiber fluorescence (TPOFF) probe and its applications for in vivo biosensing, such as real-time monitoring nanoparitcle-based drug delivery in a live mouse model.