Title:Saturated Stimulated Raman Scattering (SSRS) Microscopy for Super-resolution Vibrational Imaging: Principle, Technique, and Implementations

Speaker: Zhiwei Huang,Optical Bioimaging Lab, Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore

Date:2019-05-13 15:30

Venue:B406,New Science Building

 

Abstract: Stimulated Raman scattering (SRS) microscopy has emerged as a powerful tool for quantitative vibrational imaging of live cells and tissue with biochemical specificity, but the spatial resolution of SRS microscopy is still diffraction limited.  In this talk, I will present our very recent work on saturated stimulated Raman scattering (SSRS) microscopy to break the diffraction limit for the first time for far-field super-resolution vibrational imaging in biological systems. We have established a generic semi-classical model on SSRS processes to identify the parameters of ps/fs pump and Stokes beams required for SSRS imaging. I will discuss about a virtual sinusoidal modulation method for retrieval of super-resolution SSRS image. The first experimental implementations of SSRS microscopy for enhancing the spatial resolution of SRS imaging in biosamples are also presented by using a spectral focusing hyperspectral SRS system developed.  With the saturation occurring in coherent Raman processes under a certain level of excitation power, we expect that SSRS technique has ability of becoming an appealing quantitative imaging tool for label-free super-resolution vibrational imaging in biological and biomedical systems.